普通菜豆抗菜豆象性状的全基因组关联分析

Genome-wide Association Analysis of Resistance to Acanthoscelides obtectus in Common Bean

普通菜豆(Phaseolus vulgaris)具有丰富的营养价值,菜豆象(Acanthoscelides obtectus)是危害菜豆的主要害虫,利用抗虫种质资源防治菜豆象是最安全且经济有效的方法。该研究利用改良的室内人工接虫方法,对625份普通菜豆种质资源进行2次菜豆象抗性重复鉴定,筛选出2份抗性稳定且种子受害率均在10%以下的高抗种质。利用种子受害率和蛀孔总数的表型数据,基于3767432个SNP标记进行全基因组关联分析,鉴定出15个与种子受害率相关的显著关联遗传位点,8个与蛀孔总数相关的显著关联位点,解释了4.54%–5.56%的表型变异。在候选位点筛选出包括编码蛋白酶抑制剂、凝集素和过氧化物酶等在内的20个与抗虫防御相关的候选基因。

Common beans(Phaseolus vulgaris)have rich nutritional value,and Acanthoscelides obtectus are major pests during the storage.It is a safe,economical and effective method to control the damage of A.obtectus by screening the germplasm resources of bruchid resistance.In this study,a total of 625 common bean germplasms were identified for resistance to A.obtectus by using improved indoor artificial inoculation method.Among them,two germplasms showed stable resistance in both two repeated identifications,with the percentage of damaged seeds less than 10%.Genome-wide association analysis was conducted based on 3767432 SNP and phenotypic data of the percentage of damaged seeds and the number of perforations from 625 common beans.Fifteen significant QTLs were associated with the percentage of damaged seeds and 8 significant QTLs were associated with the number of perforations,explaining 4.54%to 5.56%of the phenotypic variation.A total of 20 candidate genes were screened from all loci,including the genes encoding protease inhibitors,lectin,peroxidase and so on,which were related to the defense of bruchids.