两种南瓜再生体系建立的方法

Regeneration System of Pumpkin: Two Establishment Methods

为建立不同途径的南瓜再生体系,以’大果蜜本’南瓜种子子叶节和授粉后2天的子房为外植体,对南瓜愈伤组织和胚状体发育两种再生途径进行研究。结果表明:愈伤组织发育途径中,3%的次氯酸钠溶液消毒15 min是最佳灭菌方式,MS+3.0 mg/L6-BA+0.1 mg/L NAA是诱导南瓜子叶节丛生芽形成的最佳培养基,MS+0.2 mg/L NAA是诱导单芽成苗的最佳培养基;胚状体发育途径中MS+4.0 mg/L 2,4-D+0.5 mg/L NAA+1.0 mg/L 6-BA和MS+0.06 mg/L TDZ均能有效诱导南瓜胚状体形成,MS培养基是诱导胚状体成苗最佳培养基。本研究成功建立了南瓜愈伤组织和胚状体发育两种途径形成再生植株的体系。

To establish different regeneration systems of pumpkin, the cotyledon nodes of pumpkin seeds and two-day ovaries after pollination of’daguo miben’were used as explants to study the two regeneration pathways of callus and embryoid development. The result showed that, in the process of callus development,3% Naclo solution for 15 minutes was the best way to sterilize callus, MS+3.0 mg/L 6-BA+0.1 mg/L NAA was the best medium to induce cluster bud formation, MS+0.2 mg/L NAA was the best medium to induce single bud seedling formation. Both MS+4.0 mg/L 2,4-D+0.5 mg/L NAA+1.0 mg/L 6-BA and MS+0.06 mg/L TDZ could effectively induce the formation of pumpkin embryoid, and MS medium was the most helpful for embryoid seedling formation. In this study, the two developmental pathways(callus and embryoid) were successfully established for regeneration systems of pumpkin.