水分胁迫对陕产重楼保护酶活性和渗透调节物质的影响

Water Stress: Effects on Protective Enzyme Activities and Osmotic Regulatory Substances of Paris polyphylla in Shaanxi

为了考察水分胁迫对陕产重楼保护酶活性和渗透调节物质含量的影响,以基质栽培的重楼种苗作为试验材料,采用质量浓度分别为5%、10%、15%的PEG-6000溶液模拟不同程度的水分胁迫,在不同处理时间取样,测定重楼叶片中保护酶活性和渗透调节物质含量。结果显示:不同浓度PEG处理重楼植株,SOD、POD活性总体呈现降低—升高—降低趋势;脯氨酸含量在胁迫2 h显著提高,15%PEG处理5天时其含量为对照组的2.61倍。可溶性糖含量在胁迫12 h增加明显,处理后期仍保持在较高水平。不同水分胁迫处理2 h,MDA迅速积累,10%PEG处理5天的含量相比对照组提高229.9%。陕产重楼具有一定的抗旱性,通过提高体内保护酶活性和渗透调节物质含量,以适应轻度水分胁迫。

To investigate the effects of water stress on the protective enzyme activity and osmotic regulatory substance content of Paris polyphylla in Shaanxi Province,the seedlings cultivated in substrate were used as experimental materials,the polyethylene glycol-6000(PEG-6000)solutions with concentrations of 5%,10%and 15%were used to simulate different water stress gradients,and the leaves of P.polyphylla were sampled at different processing stages to determine the protective enzyme activity and osmotic regulatory substance content.The results showed that,after the seedlings of P.polyphylla treated with different concentrations of PEG solutions,the activities of superoxide dismutase(SOD)and peroxidase(POD)decreased initially,followed by an increase,and then a decrease.The content of proline increased significantly after 2 h treatments,which was 2.61 times that of the control when treated with 15%PEG for 5 d.The soluble sugar content increased significantly after 12 h treatments,and remained at a high level during the later stage of treatments.Malondialdehyde(MDA)accumulated rapidly after different water stress treatments for 2 h,and the content of MDA was 229.9%higher than that of the control group on the 5 thday of 10%PEG treatment.P.polyphylla has a certain degree of drought resistance,and it can adapt to mild water stress by increasing the activity of protective enzymes and the content of osmotic regulatory substances.