LC-Q-TOF-MS和LC-IT-MS分析参麦注射液中化学成分

Analysis of chemical constituents in Shenmai Injection by LC-Q-TOF-MS and LC-IT-MS

采用LC-Q-TOF-MS和LC-IT-MS对参麦注射液中的化学成分进行定性分析。以Agilent Zorbax SB-C18(4.6 mm×250 mm,5μm)为色谱柱,0.05%甲酸水-乙腈为流动相进行梯度洗脱,流速为0.6 mL·min-1,柱温为30℃。在负离子模式下采集参麦注射液中成分的质谱数据。通过分析质谱数据、与对照品比对以及参考相关文献报道对参麦注射液中化学成分的结构进行推测和鉴定。共推测了参麦注射液中64个成分的结构,其中有16个成分经过与对照品比对确认其结构。来源于红参的56个化合物中,34个属于原人参二醇型皂苷,19个属于原人参三醇型皂苷,1个齐墩果烷型三萜皂苷和2个其他苷类成分。来源于麦冬的8个化合物中,7个甾体皂苷类化合物,1个单萜苷类化合物。该研究结果可为参麦注射液质量标准的提升和药效物质发现提供重要的理论依据。

The chemical constituents in Shenmai Injection(SMI)were qualitatively analyzed by using liquid chromatography/quadrupole time-of-flight mass spectrometry(LC-Q-TOF-MS)and liquid chromatography-ion trap-mass spectrometry(LC-IT-MS).The analysis was performed on an Agilent Zorbax SB-C18(4.6 mm×250 mm,5μm)and gradient elution was carried out with 0.05%formic acid solution-acetonitrile as mobile phase at a flow rate of 0.6 mL·min-1 and a column temperature of 30℃.Mass spectrometry data of the components in SMI were collected in negative ion mode.The structures of components were speculated and identified by analyzing mass spectrometry data,comparing with standards,and referring to related literature.A total of 64 components in SMI were estimated,and the structures were confirmed in 16 of them by comparison with standards.Fifty-six compounds derived from Ginseng Radix et Rhizoma Rubra included 34 protopanaxadiol ginsenosides,19 protopanaxatriol ginsenosides,1 oleanane ginsenosides and 2 other glycosides.Eight compounds derived from Ophiopogonis Radix included 7 steroidal saponins,and 1 monoterpene glycoside.The results of this study would provide an important theoretical basis for the improvement of the quality control standards and the discovery of effective constituents in SMI.