基于大鼠在体循环肠灌流模型研究不同粒径天麻粉的肠吸收动力学

Intestinal absorption properties of Gastrodiae Rhizoma powder with different particle size by circulation pass perfusion model in rats

为研究不同粒径天麻粉在大鼠体内的吸收特性。该研究基于大鼠在体循环肠灌流模型,建立超高效液相色谱串联质谱法(UPLC-MS/MS)测定不同粒径天麻粉中各成分的累积吸收量,考察不同粒径天麻粉、同一粒径不同质量浓度、不同肠段和胆汁对天麻素等成分的肠吸收的影响,阐明天麻粉中天麻素等成分吸收的特点,并比较不同粒径天麻粉中天麻素等成分的吸收差异。实验结果表明天麻素在各个肠段的吸收没有显著性差异,提示天麻素吸收可能为被动吸收,巴利森苷类化合物吸收为主动吸收。其中天麻素在超微粉中的吸收较好于细粉、极细粉,在高浓度下,巴利森苷类化合物的吸收在超微粉中较好,天麻粉中天麻素等各成分吸收均受到胆汁的影响。因此,适宜的超微粉碎能够提高天麻粉中有效成分的吸收,能为超微粉碎技术应用于天麻的深层次开发提供试验依据。

This study is to study the absorption properties of different particle size of Gastrodiae Rhizoma powder in rats.In vivo circulation pass perfusion model combined with ultra high performance liquid chromatography-mass spectrometry(UPLC-MS/MS)method was used to determine the cumulative absorption of each component in different particle size of Gastrodiae Rhizoma powder,and the effect of different particle size,different concentrations,different intestine segments and bile on the intestine absorption of gastrodin and other compositions in Gastrodiae Rhizoma powder was investigated to illuminate the absorption properties and compare the absorption difference of gastrodin and other compositions in Gastrodiae Rhizoma powder in different particle size.The results showed that the absorption of gastrodin in each intestinal segment has no significant difference,pointing out that gastrodin may be passive absorption and the absorption of barrison glycosides may be active absorption;the absorption of gastrodin in ultrafine powder was better than that of common powder and superfine powder of Gastrodiae Rhizoma;the absorption of these barrison glycosides was good in ultrafine powder of Gastrodiae Rhizoma under the high concentration.However,an appropriate degree of superfine grinding can promote the absorption of active ingredients of Gastrodiae Rhizoma.This test can provide information for the deep development of Gastrodiae Rhizoma.