外周血细胞因子在成人乙型病毒性肝炎疫苗长期免疫疗效中的差异研究

The role of peripheral blood cytokines in long-term immune efficacy of hepatitis B vaccine in adults

目的探索外周血细胞因子在成人乙型病毒性肝炎(简称乙肝)疫苗长期免疫疗效中的差异。方法选取免疫前乙肝表面抗原(hepatitis B surface antigen,HBsAg)、乙肝表面抗体(anti-HBs)、乙肝核心抗体(anti-HBc)均为阴性的成人乙肝疫苗免疫后8年的血清作为研究对象,用Bio-Plex悬浮芯片进行检测,分析比较无应答组(anti-HBs<10U/L)和应答组(anti-HBs≥10U/L)细胞因子浓度差异。结果应答组的干扰素诱导蛋白-10(interferon-induced protein-10,IP-10)和趋化因子(RANTES)显著高于无应答组,两组相比差异有统计学意义(Z=-2.015、-2.073,P均<0.05)。应答组的IL-10和单核细胞趋化因子-1(monocyte chemoattractant protein-1,MCP-1)显著低于无应答组,两组相比差异有统计学意义(Z=-1.981、-2.530,P均<0.05)。IL-10与MCP-1与免疫后8年抗体滴度水平呈负相关(P=0.029,P=0.032),IP-10与免疫后8年抗体滴度水平呈正相关(P=0.01)。结论IP-10、IL-10和MCP-1在调节乙肝疫苗长期免疫机制中可能起着重要作用。

Objective To explore the role of peripheral blood cytokines in the long-term immune efficacy of hepatitis B vaccine in adults.Methods Eight years after hepatitis B vaccination,blood samples were collected from participants aged 16-59 years with negative HBsAg,anti-HBs and anti-HBc before vaccination,and serum cytokine levels were determined using Bio-Plex suspension chip method.The differences in cytokine levels between the no-response group(anti-HBS<10 U/L)and the response group(anti-HBS≥10 U/L)were compared.Results The serum levels of interferon-induced protein-10(IP-10)and RANTES were significantly higher in the response group than those in non-response group(Z=-2.015,-2.073,P<0.05),while the levels of IL-10 and monocyte chemoattractant protein-1(MCP-1)were significantly lower(Z=-1.981,-2.530,P<0.05).Eight years after vaccination,IL-10 and MCP-1 levels were negatively correlated with the antibody titer(P=0.029,P=0.032),while IP-10 was positively correlated(P=0.01).Conclusions IP-10,IL-10 and McP-1 may play important roles in regulating the long-term immune mechanism of hepatitis B vaccine.