针灸对CTX荷瘤小鼠骨髓细胞中Notch信号通路的影响

Acupuncture and moxibustion on CTX tumor-burdened the effect of Notch signaling pathway in the bone marrow cells in mice

目的以Notch信号通路为切入点,通过观察针刺、艾灸对荷瘤小鼠骨髓抑制的影响,阐释Notch信号通路上差异基因与针灸改善荷瘤小鼠化疗所致骨髓抑制的信号途径及作用机制。方法选取雄性KM小鼠40只,自由喂养3天后进行植瘤,1周后,挑选荷瘤小鼠白细胞接近正常范围,瘤体大小均匀,长势良好的32只小鼠随机分为4组,相同剂量0.9%NaCl依次腹腔注射荷瘤空白组小鼠,其余3组小鼠1次性腹腔注射CTX 150mg/kg,停药后4h模型即成。荷瘤空白组小鼠每日依次固定不给予治疗措施,其余3组选取"大椎""膈俞""肾俞""足三里"依次施针和施灸,每日1次,连续5天。第6天在无菌操作台上取股骨和胫骨,运用免疫组化法、Western Blot法、Real-time PCR法检测各组荷瘤小鼠骨髓细胞Notch信号通路上差异蛋白numb。结果荷瘤小鼠施以针刺和艾灸干预后,与荷瘤模型组比,治疗组各小鼠骨髓细胞Notch信号通路上numb蛋白的表达含量均有所提升。结论针灸通过调节因CTX化疗所致过度激活的Notch信号通路上的numb基因,以平衡Notch信号通路的过度激活,改善骨髓造血功能,减轻骨髓抑制。

Objective Notch signaling pathway as the breakthrough point,through the observation of acupuncture and moxibustion for a tumor-burdened bone marrow suppression in mice,the influence of genes and explained the Notch signal pathway differences of acupuncture and moxibustion improve tumor-burdened bone marrow suppression caused by chemotherapy in mice signaling pathway and action mechanism.Methods 40 male KM mice,free feeding plant tumor after 3 days,a week later,the white blood cells close to the normal range,choose a tumor-burdened mice tumors are uniform and grew well 32 mice were randomly divided into four groups,the same dose of 0.9%Nacl,in turn,intraperitoneal injection of mice a tumor-burdened blank,the rest of the three groups of mice by intraperitoneal injection of one sex CTX 150 mg/kg,4 h later,drug withdrawal model.A tumor-burdened blank group mice were fixed in order not to give daily treatment,the remaining three groups"Da zhui","Ge shu","Shen shu","Zu sanli",needle and moxibustion,once a day,five days in a row.6 days on the stage in the femur and tibia in an aseptic operation,using immunohistochemical method and Western Blot method,the Real-time PCR method to detect each group a tumor-burdened bone marrow cells in mice Notch signaling pathway on differences in protein numb.Results The tumor-burdened mice with acupuncture and moxibustion after the intervention,and than a tumor-burdened model group,treatment group the mice bone marrow cells,Notch signaling pathway on numb protein expression levels were increased.Conclusion Acupuncture by adjusting the chemotherapy with CTX caused by excessive activation of Notch signaling pathway numb on genes,and to balance the excessive activation of Notch signaling pathways,improve bone marrow hematopoietic function,reduce the bone marrow suppression.