摘要
以羊蹄甲果荚为原料,乙醇为提取溶剂,通过单因素和正交试验对回流提取羊蹄甲果荚总黄酮工艺进行优化;并通过考察羊蹄甲果荚总黄酮对1,1-二苯基-2-三硝基苯肼(DPPH·)和羟基自由基(·OH)的清除能力来评价其抗氧化性。结果表明,羊蹄甲果荚总黄酮的最佳提取工艺参数为:提取时间5 h、料液比1:30(g:mL)、提取温度80℃、乙醇体积分数80%。在此优化工艺条件下,提取液中总黄酮含量为10.837 mg·g^-1。羊蹄甲果荚总黄酮对DPPH自由基(IC50=34.511μg·mL^-1)和羟基自由基(IC50=0.090mg·mL^-1)具有一定的清除作用,表明其具有一定的体外抗氧化活性。
Using mature seedpods of Bauhinia purpurea as raw material,and ethanol as extracting solvent,based on the results of single factor tests,the conditions of reflux extraction of total flavonoids in seedpods of Bauhinia purpurea were optimized by orthogonal tests.The antioxidant activity of total flavonoids from seedpods of Bauhinia purpurea were evaluated by investigating its scavenging capacity for 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·)and hydroxyl free radica(·OH).The best extraction process parameters of total flavonoids were determined as follows:the ethanol concentration 80%,the extraction temperature 80℃,the ratio of solid to liquid 1:30,the extraction time 5 h.Under these extraction conditions,the contents of total flavonoids in the extract was 10.837 mg·g^-1.The antioxidant tests results indicated that scavenging capacity(IC50)of total flavonoids towards DPPH·and·OH were 34.511μg·m^L-1 and 0.090 mg·mL^-1,respectively,which indicated that the total flavonoids had strong antioxidant activity.
作者
张鹏
张蝶
唐森
谢济运
ZHANG Peng;ZHANG Die;TANG Sen;XIEJi-yun(College of Food and Biochemical Engineering,Guangxi Science&Technology Normal University,Guangxi Labin 546119,China;Key Laboratory for Research and Development of Characteristic Yao Medicine Resources,Guangxi Science&Technology Normal University,Guangxi Laibin 546119,China)
出处
《当代化工》
CAS
2019年第12期2717-2722,2726,共7页
Contemporary Chemical Industry
基金
2018-2020年广西本科高校食品科学与工程特色专业及实验实训教学基地(中心)建设项目(桂教高教[2018]52号).
关键词
羊蹄甲
总黄酮
正交试验
提取工艺
抗氧化性
Bauhinia purpurea
Total flavonoids
Orthogonal test
Extraction process
Antioxidant activity