优宁八味散对地塞米松诱导大鼠非酒精性脂肪肝的保护作用及机制研究

Protective Effect and Mechanism of Youning Bawei Powder on Dexamethasone Induced Non-alcoholic Fatty Liver Disease in Rats

目的探讨蒙药优宁八味散(Mongolian Medicine Youning Bawei Powder,MMYBP)对非酒精性脂肪肝(Non-alcoholic fatty liver disease,NAFLD)模型大鼠肝脏的保护作用及其机制。方法将SD大鼠随机分为正常组,模型组,易善复组,MMYBP高、低剂量组。正常组采用普通饲料喂养,其余4组给予地塞米松注射并采用高脂饲料喂养,建立NAFLD模型,对NAFLD模型给予易善复、MMYBP高剂量和低剂量治疗1个月,检测各组肝指数、肝组织病理改变情况;酶联免疫吸附测定(Enzyme linked immunosorbent assay,ELISA)法测定血清谷丙转氨酶(Alanine aminotransferase,ALT)、谷草转氨酶(Aspartate aminotransferase,AST)、甘油三酯(Triglyceride,TG)和肝组织甘油三酯(TG)、丙二醛(Malondialdehyde,MDA)、超氧化物歧化酶(Superoxide dismutase,SOD)、游离脂肪酸(Free fatty acid,FFA)等相关指标;反转录PCR(RT-PCR)法测定肝组织CYP2E1 mRNA表达水平,蛋白质印迹法测定肝组织细胞色素P4502E1(Cytochrome P4502E1,CYP2E1)蛋白表达水平。结果MMYBP高剂量组方面,肝指数明显低于模型组(P<0.05),血清AST、ALT、TG含量显著低于模型组(P<0.05),肝组织TG、FFA和MDA含量较模型组显著降低(P<0.05),肝脏病理改变较模型组明显改善,肝组织中CYP2E1蛋白及CYP2E1 mRNA表达水平较模型组明显降低。结论MMYBP能降低NAFLD脂质代谢相关CYP2E1蛋白及CYP2E1 mRNA的表达水平,改善肝功能及脂质代谢紊乱,对NAFLD有降脂保肝的作用,延缓NAFLD向肝纤维化及肝硬化进展。

Objective To investigate the protective effect of Mongolian Medicine Youning Bawei Powder(MMYBP)on the liver of non-alcoholic fatty liver disease(NAFLD)model rats and its mechanism.Methods SD rats were randomly divided into normal group,model group,Yi Shan Fu group,MMYBP high and low dose groups.The normal group was fed with ordinary diet,and the other four groups were injected with dexamethasone and fed with high-fat diet,then establish NAFLD model.NAFLD model was treated with Yi Shan Fu,MMYBP high dose and low dose for one month.The liver index and pathological changes of liver tissue were detected.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG)and liver triglyceride(TG),malondialdehyde(MDA),superoxide dismutase(SOD)and free fatty acid(FFA)indexes were measured by enzyme linked immunosorbent assay(ELISA).The expression level of CYP2E1 mRNA in liver tissue was measured by reverse transcription PCR(RT-PCR).The expression of cytochrome P4502E1(CYP2E1)protein in liver tissue was measured by Western blot.Results The liver index of MMYBP high dose group was significantly lower than that of model group(P<0.05).The contents of AST,ALT and TG in serum were significantly lower than those in model group(P<0.05).The contents of TG,FFA and MDA in liver tissue were significantly lower than those in model group(P<0.05).The pathological changes of liver were significantly improved compared with the model group.The expression levels of CYP2E1 protein and CYP2E1 mRNA in liver tissue were significantly lower than those in model group.Conclusion MMYBP can reduce the expression level of CYP2E1 protein and CYP2E1 mRNA related to lipid metabolism in NAFLD,improve liver function and lipid metabolism disorder,reduce lipid and protect liver in NAFLD,and delay the progression of NAFLD to liver fibrosis and cirrhosis.