基于网络药理学的藏药西红花“治一切肝病”的药理作用分析

Pharmacological analysis of Tibetan Medicine Crocus sativus L.(saffron) on the Liver Disease Based on Network Pharmacology

目的在藏医药理论体系中,西红花为"治一切肝病"的药,本文通过网络药理学的研究方法,探讨藏药西红花防治肝病的临床应用范围及作用机制。方法根据西红花成分的专属性、生物利用度及相关文献报道筛选目标化合物。利用PharmMaper数据库对目标化合物开展靶点预测分析,建立"成分-靶点"网络,开展网络拓扑分析;并进行PPI分析、KEGG通路富集等生物功能注释;通过OMIM、Disgenet数据库获取肝病相关基因,建立"成分-靶点-疾病"网络。结果筛选出19个西红花的目标化合物,获得潜在靶点80个;经生物功能注释分析发现与肝癌、肝纤维化、乙型肝炎、非酒精性肝病等七种肝病相关度较高的通路有PI3K-Akt、MAPK等12条。推测西红花防治肝病的可能关键靶点为SRC和EGFR。结论初步明确西红花在防治肝病的临床应用范围及可能的作用机制,为丰富完善藏医药理论开发肝病新药提供了科学支撑。

Objective To elucidate the mechanism of Crocus sativus L.(saffron)in the prevention and treatment of liver disease by network pharmacology and investigate the relationship between Sino-Tibetan medicine in the use of saffron.Methods Using oral utilization,bioavailability and pharmacology studies as the limiting conditions,the components of saffron were screened,the related targets were predicted using PharmMaper server,and the"components-targets"network was established for the network analysis.OMIM and Disgenet databases were used to screen hepato-related genes and protein targets;the KEGG pathway was used to enrich the selected targets;the network of"componentstargets-disease"was obtained by PPT analysis.Results 19 components were screened and 80 potential targets were predicted.Molecular docking results showed that 12 pathways including PI3 K-AKT,MAPK are highly hepato-related.Among them,SRC and EGFR are the key targets.Conclusion The mechanism of the saffron in the prevention and treatment of liver disease was discussed.It will provide theoretical basis for the clinical application of Tibetan medicine in the future.