基于维生素D免疫调节作用研究加味凉膈散在脓毒症急性肺损/急性呼吸窘迫综合征中的干预机制

Research on intervention mechanism of modified Liangge Powder in sepsis acute lung injury/acute respiratory distress syndrome based on immune regulation of vitamin D

目的:基于维生素D免疫调节作用探讨加味凉膈散(LGS)对盲肠结扎穿孔术(CLP)诱导的脓毒症急性肺损伤(ALI)/急性呼吸窘迫综合征(ARDS)小鼠的干预机制。方法:将72只8周龄雄性C57小鼠随机分为假手术组、模型组、LGS组。连续给药5 d,末次给药1 h后,采用CLP建立脓毒症ALI/ARDS模型。每组各取12只,观察小鼠的7 d生存率;剩余小鼠于术后24 h处死,收集血清,采用酶联免疫吸附法(ELISA)检测炎症因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及25-羟基维生素D3[25(OH)D3]水平。结果:①与假手术组相比,模型组小鼠7 d生存率显著降低(P<0.05),血清中炎症因子IL-6、TNF-α水平显著升高,而25(OH)D3水平显著降低(P<0.05);②与模型组比较,LGS组小鼠7 d生存率显著提高(P<0.05),血清中炎症因子IL-6、TNF-α水平显著降低(P<0.05),25(OH)D3水平明显升高(P<0.05)。结论:LGS可能基于维生素D免疫调节作用干预脓毒症ALI/ARDS。

Objective:To explore the intervention mechanism of modified Liangge Powder(LGS)in sepsis-associated acute lung injury(ALI)/acute respiratory distress syndrome(ARDS)mice based on the immune regulation of vitamin D.Methods:Seventy-two 8-week-old male C57 mice were randomly divided into sham operation group,model group and LGS group.The drugs were administered continuously for 5 days,then the sepsis-associated ALI/ARDS model was established 1 h after the last administration by CLP.Twelve mice in each group were selected to observe the 7-day survival rate.The remaining mice were killed 24 h after operation,and the serum was collected.The inflammatory factors interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and 25-hydroxyvitamin D3[25(OH)D3]were detected by enzyme linked immunosorbent assay(ELISA).Results:①Compared with the sham operation group,the 7-day survival rate of the model group was significantly decreased(P<0.05),the serum levels of inflammatory factors IL-6 and TNF-α were significantly increased(P<0.05),and the level of 25(OH)D3 was significantly decreased(P<0.05).②Compared with the model group,the 7-day survival rate of was significantly improved(P<0.05),the serum levels of inflammatory factors IL-6 and TNF-α were significantly decreased(P<0.05),and level of 25(OH)D3 was significantly increased(P<0.05)in the LGS group.Conclusion:LGS may intervene the sepsisassociated ALI/ARDS based on the immune regulation of vitamin D.