266例生殖道沙眼衣原体与解脲支原体标本结果及其与不孕症的关系

266 cases of genital tract Chlamydia trachomatis and Ureaplasma urealyticum specimens and their results relationship to infertility

目的分析生殖道沙眼衣原体(CT)以及解脲支原体(UU)标本行荧光定量PCR的检测结果,及其与不孕症的关系。方法选择广州医科大学附属顺德医院于2018年1月-2020年12月收治的266例生殖道沙眼衣原体、解脲支原体感染标本为研究组,选择同一时间在本院进行体检的健康女性266名为对照组,对所有受试者均采用荧光定量聚合酶链反应(FQ-PCR)进行检测,并对比沙眼衣原体与解脲支原体的感染特点以及检出情况。结果两组受试者年龄、体质质量以及孕次比较,差异无统计学意义(P>0.05)。荧光定量检查显示,研究组CT阳性检出率为44.74%(119/266),UU阳性检出率为36.09%(96/266);对照组CT阳性检出率为6.77%(18/266),UU阳性检出率为3.76%(10/266)。两组CT以及UU阳性检测结果比较,差异有统计学意义(P<0.05)。不孕组与继发性不孕组、正常组UU阳性检出率比较,差异有统计学意义(χ^(2)=5.687、4.104,P<0.05);不孕组与流产组UU阳性检出率比较,差异无统计学意义(χ^(2)=0.035,P>0.05)。继发性不孕组与流产组UU阳性检出率比较,差异无统计学意义(χ^(2)=2.628,P>0.05);继发性不孕组与正常组UU阳性检出率比较,差异有统计学意义(χ^(2)=13.128,P<0.05)。流产组与正常组UU阳性检出率比较,差异无统计学意义(χ^(2)=0.862,P>0.05)。不孕组与正常组、流产组CT阳性检出率比较,差异有统计学意义(χ^(2)=10.377、10.377,P<0.05)。继发性不孕组与正常组CT阳性检出率比较,差异有统计学意义(χ^(2)=8.507,P<0.05);继发性不孕组与流产组CT阳性检出率比较,差异无统计学意义(χ^(2)=0.588,P>0.05);流产组与正常组CT阳性检出率比较,差异无统计学意义(χ^(2)=1.623,P>0.05)。结论采用FQ-PCR对生殖道感染患者进行检查,CT以及UU的数据变化能够为临床确诊不明原因的不孕不育症患者提供参考,值得将其作为常规检查进行推广。

Objective To analyze the results of fluorescence quantitative PCR in 266 specimens of genital Chlamydia trachomatis(CT)and Ureaplasma urealyticum(UU).Methods266 cases of genital Chlamydia trachomatis and Ureaplasma urealyticum infection in the Affiliated Shunde Hospital of Guangzhou Medical University from January 2018 to December2020 were selected as the study group,and 266 healthy women who underwent physical examination in the Affiliated Shunde Hospital of Guangzhou Medical University at the same time were selected as the control group.All subjects were detected by fluorescence quantitative polymerase chain reaction(FQ-PCR).The infection characteristics and detection of CT and UU were compared.ResultsThere was no significant difference in age,constitution and pregnancy between the two groups(P>0.05).Fluorescence quantitative examination showed that the positive detection rate of CT in the study group was 44.74%(119/266),the positive detection rate of UU was 36.09%(96/266);the positive detection rate of CT in the control group was 6.77%(18/266)and UU positive detection rate was 3.76%(10/266).There were statistically significant differences in CT and UU positive test results between the two groups(P<0.05).Compared with the secondary infertility group and the normal group,the UU positive detection rate of the infertility group was significantly different(χ^(2)=5.687,4.104,P<0.05).The difference was not statistically significant in the positive detection rate of UU between infertility group and abortion group(χ^(2)=0.035,P>0.05).There was no significant difference in the positive detection rate of UU between the secondary infertility group and the abortion group(χ^(2)=2.628,P>0.05);there was a statistical difference in the positive detection rate of UU between the secondary infertility group and the normal group(χ^(2)=13.128,P<0.05).There was no significant difference in the positive detection rate of UU between the abortion group and the normal group(χ^(2)=0.862,P>0.05).There was a statistically significant difference in the positive detection rate of CT between the infertility group and the normal group and the abortion group(χ^(2)=10.377,10.377,P<0.05).There was a statistically significant difference in the positive detection rate of CT between the secondary infertility group and the normal group(χ^(2)=8.507,P<0.05);there was no statistical difference in the positive detection rate of CT between the secondary infertility group and the abortion group(χ^(2)=0.588,P>0.05).There was no significant difference in the positive detection rate of CT between the abortion group and the normal group(χ^(2)=1.623,P>0.05).ConclusionsFQ-PCR was used to examine patients with reproductive tract infection.The data of CT and UU could provide reference for the clinical diagnosis of unexplained infertility patients,which was worthy to be popularized as a routine examination.