SREBP2在原发性肝癌中的表达水平与临床病理特征及预后的关系

Relationship between expression of SREBP2 in primary liver cancer and clinicopathological features and prognosis

目的探讨胆固醇调节原件结合蛋白2(SREBP2)在原发性肝癌中的表达情况,并分析其与临床病理特征及预后的关系。方法选取2014年1月至2016年1月于广州中医药大学第一附属医院收治的70例原发性肝癌患者作为研究对象。分别采用实时荧光定量聚合酶链反应(qRT-PCR)和免疫组织化学(IHC)检测肝癌组织和癌旁正常组织中SREBP2 mRNA和SREBP2蛋白表达情况;分析SREBP2蛋白与患者临床病理特征的关系;用KaplanMeier法分析患者生存情况并用Log-rank法比较生存率;用Cox比例风险回归模型探讨影响患者预后的危险因素。结果SREBP2 mRNA在原发性肝癌癌组织中表达水平为(4.26±0.84),高于癌旁正常组织(1.06±0.32),差异有统计学意义(P<0.05);SREBP2蛋白在原发性肝癌癌组织中阳性率为67.14%,高于癌旁正常组织25.71%,差异有统计学意义(P<0.05)。SREBP2蛋白在原发性肝癌不同病理类型、分化程度、门静脉侵袭及TNM分期等临床病理特征上差异表达有统计学意义(P<0.05);但在性别、年龄、肿瘤直径及肿瘤数量上差异无统计学意义(P>0.05)。与阴性者比较,原发性肝癌中SREBP2蛋白阳性者3年PFS和OS生存时间明显缩短、生存率明显降低,差异均有统计学意义(P<0.05)。经COX回归分析得知,有门静脉侵袭、TNMⅢ期和SREBP2蛋白阳性是影响原发性肝癌预后的独立危险因素,差异有统计学意义(P<0.05)。结论SREBP2在原发性肝癌中高表达,与病理类型、分化程度、门静脉侵袭及TNM分期等临床病理特征及预后相关,SREBP2高表达明显增加患者死亡风险。

Objective To investigate the expression of sterol-regulating element binding protein 2(SREBP2)in primary liver cancer,and analyze its relationship with clinicopathological features and prognosis.Methods Seventy patients with primary liver cancer admitted to our hospital from January 2014 to January 2016 were selected as subjects.Real-time quantitative polymerase chain reaction(qRT-PCR)and immunohistochemistry(IHC)were used to detect the expression of SREBP2 mRNA and SREBP2 protein in hepatocarcinoma tissues and adjacent normal tissues.The relationship between SREBP2 protein and clinicopathological features was analyzed by test.The survival of the patients was analyzed by KaplanMeier method and the survival rate was compared by Log-rank method.The risk factors affecting the prognosis of patients were investigated by Cox proportional hazards regression model.Results The expression levels of SREBP2 mRNA in primary liver cancer tissues and adjacent normal tissues were(4.26±0.84)and(1.06±0.32),respectively;the difference was statistically significant(P<0.05).SREBP2 protein was found in primary liver cancer.The positive rates in tissues and adjacent normal tissues were 67.14%and 25.71%,respectively,and the difference was statistically significant(P<0.05).SREBP2 protein was differentially expressed in different pathological types,differentiation,portal vein invasion and TNM staging of primary hepatocellular carcinoma(P<0.05),but there were on significant difference in gender,age,tumor diameter and tumor number(P>0.05).Compared with the negative ones,the survival time of progress free survival(PFS)and overall survival(OS)in the SREBP2 protein positive patients in primary liver cancer was significantly shorter(P<0.05),and the survival rate was significantly lower(P<0.05).According to COX regression analysis,portal vein invasion,TNM stageⅢand SREBP2 protein positive were independent risk factors for the prognosis of primary liver cancer(P<0.05).Conclusions SREBP2 was highly expressed in primary liver cancer,which was associated with pathological type,differentiation,portal vein invasion and TNM staging.The high expression of SREBP2 significantly increases the risk of death.