桔梗提取物对类风湿性关节炎大鼠的抗炎作用

Anti-inflammatory Effects of an Extract from Platycodon grandiflorum on Rats with Rheumatoid Arthritis

探究桔梗提取物对类风湿性关节炎大鼠关节肿胀的干预效果及抗炎作用。选取55只SD大鼠,5只作正常组,其余50只建立类风湿性关节炎模型,分为模型组、药物对照组、低、中、高浓度组,分别干预。观察各组大鼠足体积、关节炎指数及丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、白细胞介素-8(IL-8)、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)水平。结果显示,建模后第1、7、14、20 d,高浓度组大鼠足体积分别为2.12 mL、2.60 mL、2.93 mL、2.49 mL,关节炎指数分别为2.03、2.86、5.25、4.37,低于其他四组(p<0.05);高浓度组大鼠MDA、IL-8、CRP、TNF-α水平分别为21.46μmol/L、101.33 pg/mL、4.34 mg/L、4.86 ng/mL,低于其他四组(p<0.05);SOD、GSH-Px水平分别为86.11 nU/mL、85.37 U/L,高于其他四组(p<0.05)。桔梗提取物能够减轻类风湿性关节炎大鼠关节肿胀程度,改善抗氧化能力,抑制炎症反应。

To explore the intervention and anti-inflammatory effects of an extract from Platycodon grandiflorum on joint swelling in rats with rheumatoid arthritis,fifty-five SD rats were selected,with five as the normal group,and the remaining 50 rats established as rheumatoid arthritis models(which were divided into the model group,drug control group,and low,medium and high concentration groups,respectively).The paw volume,arthritis index,and the levels of malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),interleukin-8(IL-8),C-reactive protein(CRP)and tumor necrosis factor-α(TNF-α)were measured.The results showed that:On the 1st,7th,14th and 20th day after modeling,the paw volume of the rats in the high concentration group was 2.12 mL,2.60 mL,2.93 mL and 2.49 mL,respectively,and the arthritis index was 2.03,2.86,5.25 and 4.37,respectively,which were lower than those of the other four groups(p<0.05);the levels of MDA,IL-8,CRP and TNF-αin the high concentration group were 21.46μmol/L,101.33 pg/m L,4.34 mg/L and 4.8 mg/L,respectively,and the levels of SOD and GSH-Px were 86.11 nU/mL and 85.37 U/L,respectively,which were higher than those of the other four groups(p<0.05).The extract from Platycodon grandiflorum could reduce the degree of joint swelling in rats with rheumatoid arthritis,improve antioxidant capacity while inhibiting inflammation.