竹材木质素降解菌的筛选与鉴定

Screening and Identification of Bamboo Lignin Degradation Fungus

竹材木质素的降解一直是制约竹材资源利用的重要影响因素。文章通过对49份腐烂的竹材样品进行平板划线分离纯化得到74株真菌,利用PDA-愈创木酚变色及PDA-苯胺蓝退色反应,初步筛选出具有木质素降解酶能力的4株木质素降解菌,通过酶活力测定对初筛得到的4株木质素降解菌进行复筛,发现FG-22菌株具有较好木质素降解能力,对FG-22降解菌株进行形态学和分子生物学鉴定,确定FG-22为杂色云芝(Trametes versicolor)。

The degradation of bamboo lignin has always been an important factor influencing the utilization of bamboo resources.Seventy-four fungi were isolated by streaking and purifying 49 samples of rotten bamboo.The lignin-degrading fungi producing lignin-degrading enzyme activity were screened by PDA-guaiacol phenol discoloration and PDA-aniline blue fading reaction.Four strains of lignin-degrading fungi were screened by enzyme activity assay.It was found that FG-22 fungus had better lignin degradation ability,and the FG-22-degrading fungus was identified by morphological identification and molecular biology to determine FG-22 is Trametes versicolor.