试论趋化因子CXCR4在卵巢肿瘤干细胞活动中的作用

Discussion on Chemokine CXCR4 Stem Cell Activity in Ovarian Tumors

目的通过研究卵巢干细胞中趋化因子受体在肿瘤干细胞系中侵袭、转化中的作用,探讨治疗卵巢肿瘤的新方法。方法采用流式细胞术和RT-PCR方法检测趋化因子的活动表达,同时通过体外微孔隔离室观察趋化因子受体在卵巢肿瘤细胞侵袭、转移活动中作用。结果趋化因子受体CXCR4在卵巢癌细胞中呈阳性,通过封闭CXCR4可以抑制趋化因子CXCL12族对肿瘤迁移的促进作用。结论卵巢肿瘤干细胞中趋化因子受体的活跃程度直接和肿瘤生长活动相关,发挥趋势因子的沉默作用可以减缓卵巢癌细胞的生长。

Objective Through the study of ovarian stem chemokine receptors in the tumor invasion,transformation of stem celllines cells,to explore the new method of treatment of ovarian cancer.Methods The expression of chemotactic factor detection by flow cytometry and RT-PCR method,at the same time by in vitro micro isolation room chemokine receptor invasion,metastasis in ovarian tumor cellactivity.Results The chemokine receptor CXCR4 in ovarian cancer cells were positive by blocking the CXCR4 can inhibit the chemotaxis,promoting factor CXCL12 family of tumor migration.Conclusion Ovarian tumor stem cell chemokine receptor activity and tumor growth inhibition activity,play the trend factor can slow the growth of ovarian cancer cells.