IL-10和TNF-α基因多态性与肝细粒棘球蚴感染坏死的关联性

Association of IL-10 and TNF-αgene polymorphisms with hepatic echinococcus granulosus infection and necrosis

目的探讨肝细粒棘球蚴感染坏死与IL-10、TNF-α基因多态性的关联性,明确基因层面相关影响因素。方法选取2018年1月至2020年12月在新疆维吾尔自治区人民医院肝胆外科行手术治疗的肝包虫病患者106例,将发生肝细粒棘球蚴感染所致坏死的患者作为观察组,未发生肝细粒棘球蚴感染所致坏死的作为对照组,每组各53例。抽取所有患者外周血,运用酶联免疫吸附法(ELISA)检测血清中IL-10、TNF-α水平,采用聚合酶链式反应(PCR)技术检测IL-10(-592,-1082)、TNF-α(rsl800630)多态性,分析IL-10、TNF-α水平及其基因多态性在各组的差异。结果观察组患者血清IL-10、TNF-α水平均明显高于对照组,差异有统计学意义(均P<0.05);两组间IL-10(-592,-1082)、TNF-α(rsl800630)基因多态性位点基因型与等位基因频率差异具有统计学意义(均P<0.05);观察组IL-10基因-592C/A位点CC基因型患者血清IL-10水平高于CA+AA基因型患者,差异有统计学意义(P<0.05);观察组IL-10基因-1082T>A位点TT基因型患者血清IL-10水平高于TA+AA基因型患者,差异有统计学意义(P<0.05)。观察组TNF-α基因rsl800630C/A位点CC基因型患者血清TNF-α水平高于CA+AA基因型患者,差异有统计学意义(P<0.05)。结论IL-10(-592,-1082)、TNF-α(rsl800630)基因多态性位点改变与肝细粒棘球蚴感染坏死具有关联性。

Objective To investigate the association between hepatic echinococcus granulosus infection and necrosis with gene polymorphism of interleukin-10(IL-10)and tumor necrosis factor-α(TNF-α),and to identify the related factors at the gene level.Methods A total of 106 patients with hepatic echinococcosis who underwent surgical treatment in the department of hepatobiliary surgery,People′s Hospital of Xinjiang Uygur Autonomous Region from January 2018 to December 2020 were selected.Patients with necrosis caused by hepatic echinococcus granulosus infection were selected as the observation group,and patients without necrosis caused by hepatic echinococcus granulosus infection were selected as the control group,with 53 cases in each group.The serum levels of IL-10 and TNF-αwere detected by enzyme-linked immunosorbent assay(ELISA).The polymorphisms of IL-10(-592,-1082)and TNF-α(rsl800630)were detected by polymerase chain reaction(PCR).The levels of IL-10 and TNF-αand their gene polymorphisms were analyzed.Results The levels of serum IL-10 and TNF-αin the observation group were significantly higher than those in the control group(all P<0.05);There was significant difference in genotype and allele frequency of IL-10(-592,-1082)and TNF-α(rsl800630)(all P<0.05).The serum IL-10 level of CC genotype patients with IL-10 gene-592C/A locus in the observation group was higher than that of CA+AA genotype patients,with statistically significant difference(P<0.05).The serum IL-10 level in patients with TT genotype at-1082T>A of IL-10 gene in the observation group was higher than that in patients with TA+AA genotype,with statistically significant difference(P<0.05).The serum TNF-αlevel in patients with CC genotype at rsl800630C/A locus of TNF-αgene in the observation group was higher than that in patients with CA+AA genotype,with statistically significant difference(P<0.05).Conclusions The changes of IL-10(-592,-1082)and TNF-α(rsl800630)gene polymorphisms may be associated with hepatic echinococcus granulosus infection and necrosis.