阳春砂与绿壳砂、海南砂的ITS-1测序鉴别

Identification of Amomum villosum, Amomum villosum var.xanthiaides and Amomum longiligulare on ITS-1 Sequence

目的:从分子水平鉴别阳春砂及其伪充品。方法:从阳春砂及其常见伪充品绿壳砂、海南砂中提DNA,以核基因组通用引物ITS-1为引物进行扩增,扩增产物经纯化后,用PCR产物直接测序法进行测序。结果:样品ITS-1序列长度均为248bp,但绿壳砂有7个碱基与阳春砂不同,海南砂有12个碱基与阳春砂不同。结论:1序列可有效地鉴别阳春砂及其伪充品。

Objective:To identify Amomum villosum Lour, and some their adulterants on molecular biology.Method:The DNA of Amomum villosum Lour, and some their adulterants were extracted, and amplified using ITS-1 primer.The amplificed DNA were purified and then se-quenced by direct PCR sequencing method. Result: The ITS-sequence of all of the samples are 248 bp in size.But there are 7 bases in Amomum villosum Lour var. xanthioides (Wall.ex Bak) T.L.Wu et Senjen and 12 bases in Amomum longiligulare T.L.Wu.differing from...