摘要
采用最佳工艺条件水煮提取山药多糖,醇沉干燥得到粗多糖DT,DT再经过DEAE-Sepharose CL-6B 柱层析分离纯化后得2个组分,分别命名为DTA和DTB。采用葡聚糖凝胶G-100柱层析和醋酸纤维素薄膜纸电泳检测DTA的纯度,实验显示:DTA为单一多糖,利用高效液相色谱分析DTA的组成。结果表明,DTA 由果糖和葡萄糖2种单糖组成,摩尔比为:1:26.36。
A water-soluble crude polysaccharide DT was obtained after extracting in optimal conditions, concentrating, precipitating with 4 times volume of absolute alcohol and desiccating. Two water-soluble polysaccharides named DTA and DTB were obtained by DEAE-Sepharose CL-6B chromatogra-phy. Purity detection indicated that DTA was homogeneous by gel filtration chromatography on Sephadex G-100 and cellose acetate pellicle electrophoresis. The monosaccharides of DTA were analyzed by using HPLC, the result showed tha...
出处
《福建中医学院学报》
2006年第3期40-43,共4页
Journal of Fujian College of Traditional Chinese Medicine
关键词
山药
纯化
高效液相色谱
Diascorea opposite Thunb
purification
HPLC