水分胁迫下马铃薯试管苗的生理响应

Physiological response of test-tube plantlets of potato under water stress

利用PEG-6000模拟根际水分胁迫法,对马铃薯试管苗进行了水分胁迫处理,研究马铃薯不同品种在不同水分胁迫程度下的生理反应。结果表明,马铃薯试管苗体内丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性与可溶性蛋白质含量在水分胁迫下发生了不同的变化。其中,在轻度水分胁迫(10%PEG)下各马铃薯品种可溶性蛋白含量均上升,脯氨酸大量积累,MDA含量急剧增加,POD、SOD、CAT活性上升;经重度水分胁迫(20%PEG)后,以上各指标变化趋势不一。马铃薯不同品种对水分胁迫的反应敏感程度不一致,这与其不同的抗旱能力有关。

Test-tube plantlets of potato were treated with water stress by PEG-6000, and then, the physiological response of different cultivars under different degree of water stress were analyzed. The results showed that the content of MDA, proline and soluble protein, activities of SOD, POD and CAT changed defferenrly with different water stress. Under light water stress ( 10 % PEG ), the content of soluble protein increased, a great quantity of proline were accumulated, content of MDA was increased steeply, the ac...