摘要
目的探讨痰液中白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、中性粒细胞与吸烟者气道炎症的相关性,以及IL-8、TNF-α、中性粒细胞与吸烟的相关性。方法收集30例男性吸烟者(吸烟组)和30例男性非吸烟者(非吸烟组)的痰液,对痰液进行痰细胞分类及计数,并用酶联免疫吸附法(ELISA)测定痰上清液中IL-8、TNF-α的浓度。结果吸烟组痰液中IL-8水平、中性粒细胞百分比明显高于非吸烟组,差异均有统计学意义(分别为P<0.05,P<0.01);吸烟组痰液中TNF-α水平与不吸烟组比较,差异无统计学意义(P>0.05)。吸烟组中,吸烟指数>400支·年者与吸烟指数≤400支·年者比较,痰上清液中IL-8水平差异无统计学意义(P>0.05)。吸烟组痰液中IL-8水平与中性粒细胞百分比呈正相关性(r=0.544)。结论IL-8和中性粒细胞共同参与了吸烟者气道炎症反应,吸烟是导致气道炎症的一种重要因素。
Objective To evaluate the relationship between respiratory tract inflammation and interleukin-8,tumor necrosis factor-α,neutrophil in the sputum and the relationship between these factors and smoking in smokers.Methods 30 healthy smokers and 30 healthy nonsmokers were chosen.The sputum samples were collected by inducing method.Cells in the sputum were examined by microscopy.The levels of interleukin-8 and tumor necrosis factor-α in fluid-phase of sputum were determined by enzyme-linked immunosorbent assay(E...
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2007年第3期159-161,共3页
Journal of Environment and Health
关键词
呼吸系统
吸烟
炎症
Respiratory system
Smoking
Inflammation