10种卷柏属药用植物的红外光谱鉴别及部分种间亲缘关系探讨

FTIR Identification of the 10 Species of Selaginella and Relationships Discussion among the Part Species

利用红外光谱技术对卷柏属10种药用植物进行了比较鉴定研究.结果表明,10种植物样品通常具有10个共有红外吸收峰,但又具有各自的特征吸收峰(卷柏、垫状卷柏、疏叶卷柏、细叶卷柏、异穗卷柏等),或所有吸收峰的组合具有种间特征性.可利用整体峰形及吸收峰的数目、位置(cm^(-1))、强度等特征,尤其是指纹区(1 250～400 cm^(-1))的上述特征,对各个种进行有效的鉴别.根据光谱图的相似程度,推测异穗卷柏Selaginella heterostachys、细叶卷柏S.labordei及伏地卷柏S.nipponica之间具有较密切的亲缘关系,江南卷柏S.maellendorffii与中华卷柏S.sinensis的关系也较接近;并结合形态解剖学等方面的差异,提出将薄叶卷柏的变异类型作为它的一个变种处理.研究证实,红外光谱操作简便、快速,可有效辅助用于近缘植物种的分类鉴定,辅助解决鉴定中的某些疑点问题,推断部分种间的亲缘关系.

Comparative identification of the 10 Chinese medicinal species of Selaginella by IR was performed.The FTIR spectra obtained show that there are generally 10 common absorption peaks in all the species,but each species has its particular characteristic pattern as a whole.All species (including S.tamariscina,S.pulvinata,S. remotifolia,S.labordei,S.heterostachys etc.) can be differentiated from each other according to their infrared spectral patterns.The wave-number (λ_(max)) and the height of the absorption pe...