低剂量辐射后小鼠骨髓白血病细胞凋亡的观察

Apoptosis of Bone Marrow Leukemia Cells in Mice after Low Dose Radiation at Different Time

目的观察低剂量辐射(LDR)后不同时间小鼠骨髓白血病细胞凋亡的变化,探讨全身低剂量辐射辅助治疗白血病的理论依据。方法将小鼠粒单白血病细胞系WEH I-3尾静脉接种于BALB/c小鼠建立白血病动物模型。将60只成功建立的粒单白血病模型小鼠,对半分为对照组和实验组。对照组小鼠不进行照射,实验组小鼠同时给予75mGy的LDR。于LDR后1d、2d、3d、5d和10d分别处死实验组及对照组6只小鼠,取其骨髓,通过荧光显微镜、电镜观测骨髓白血病细胞的凋亡情况。结果实验组小鼠LDR后第2d、3d骨髓白血病细胞凋亡率最高,5d和10d次之,与对照组比较差异显著(P均<0.05)。结论LDR可使白血病小鼠骨髓肿瘤细胞凋亡率增加,其作用机制明显不同于大剂量射线治疗对肿瘤细胞的杀伤,可能与LDR增加白血病小鼠免疫兴奋效应,促进某些细胞因子分泌有关。

Objective To investigate the apoptosis of bone marrow leukemia cell in mice after low dose radiation(LDR) at different time and the experimental basis for LDR auxiliary therapy on leukemia.Methods WEHI-3 cells were injected into BALB/c mice through tail veins to make an experimental mice model of myelomonocytic leukemia.60 leukemia mice models were divided half-and half.30 mice models in experimental group were irradiated with LDR of 75mGy at the same time while the others 30 in the control group were not.6 mice models with LDR and 6 mice models without LDR would be killed at the time the 1st day,the 2nd day,the 3rd day,the 5th day and the 10th day after LDR in order to extract bone marrow samples.The apoptosis percentage of leukemia cells in bone marrow was examined.Results The apoptosis percentage of leukemia cells in experimental group was increasing after LDR and went to top on the 2nd day and the 3rd day.The apoptosis percentage of leukemia cells was remarkably different between experimental and control group,all P<0.05.Conclusion LDR could significantly increase the apoptosis percentage of bone marrow leukemia cells in mice.Its mechanism is remarkably different in kill and wound of big dose radiation to tumour cells.It is probably related to of the increase immune exciting response as to promote some cytokine secretion.in leukemia mice.