南瓜种质资源亲缘关系的RAPD分析

Genetic Diversity Analysis of Squash Germplasm Resources with RAPD

南瓜是国内外广泛栽培的重要蔬菜作物之一,其种质资源十分丰富。开展南瓜的遗传多样性和亲缘关系的研究,有利于了解南瓜遗传多样性的分布和种质间的遗传关系,促进南瓜种质的收集和有效利用。以不同来源地、不同生态型的南瓜种质资源为试材,用CTAB方法从南瓜幼嫩叶片提取基因组DNA,用长度为10个碱基的寡核苷酸作RAPD引物进行PCR扩增反应。从260个随机引物筛选出26个多态性引物,对76份南瓜种质资源进行扩增,共扩增出255条带,其中多态性带为207条,多态性百分率为81.18%。表明南瓜种质资源遗传多样性丰富。聚类分析将供试材料分为3大类:中国南瓜、美洲南瓜和印度南瓜,种间差异明显。

Squash is one of the important worldwide vegetable crops, which is extremely variable in germplasm resource. Study on genetic diversity of squash will favor for understanding genetic diversity distribution and relationships of germplasm, and offering the theoretical help for collection and application of squash germplasm. Squash germplasm resources from different countries or regions were used as experimental material. DNA was extracted from young leaves using CTAB method. 10-base-long oligonucleotide primers were used in polymerase chain reaction. 26 polymorphic primers were screened from 260 random primers. A total of 255 bands were detected among 76 squash accessions from different countries and regions. The genetic diversity is rich. Of them 207 (81.18%) bands were polymorphic. All accessions were clustered into three groups: Cucurbita moschata、Cucurbita maxima and Cucurbita pepo. The difference within cultivars is obvious.