高效液相色谱法测定山楂叶提取物中槲皮素的含量

Determination of Quercetin in Hawthorn Leaves by HPLC

目的:用高效液相色谱法(HPLC法)测定山楂叶中槲皮素的含量.方法:采用C18柱,以甲醇:0.4%磷酸(45∶55)为流动相,流速为0.9mL/min,检测波长为360nm.结果:槲皮素在0.1624～1.624μg/mL范围内与峰面积线性关系良好,相关系数为0.9998,平均回收率为97.9%,RSD为0.59%(n=6).结论:HPLC法操作简便、准确,可作为山楂叶提取物的定量分析方法.

Objective:To develop a HPLC method for determining the content of quercetin in hawthorn leaves.Methods:The Suntek ODS column with methanol-0.4% phosphoric acid(45∶55) as the mobile phase was used.The flow rate was 0.9mL/min and the detective wavelength was 360nm.Results:The linear range of calibration curve was 0.1624～1.624μg/mL,for the quercetin(r=0.9998).The average recovery was 97.9% and the RSD was 0.59%(n=6).Conclusion:The method is simple,and accurate with a good repeatability and can be used as quantitative analysis for this preparation.