补肾健脾活血方对去卵巢大鼠骨组织TNF-α表达的影响

Effect of Bushen Jianpi Huoxue recipe on expression of TNF-α in bone tissue of ovariectomiced rats

目的 观察复方中药补肾健脾活血方对绝经后骨质疏松症大鼠模型骨组织中肿瘤坏死因子α(TNF-α)表达的影响。方法 将84只大鼠随机分为正常组、假手术组、模型组、中药对照组、西药对照组、补肾健脾活血方大、小剂量组共7组,采用卵巢切除的方法建造绝经后骨质疏松症动物模型,灌药90 d后取腰椎骨用免疫组化的方法检测TNF-α的表达。结果 补肾健脾活血方能明显减少TNF-α表达的阳性细胞数,其作用强于单纯补肾中药和西药治疗,并且与剂量呈正相关。结论 补肾健脾活血方通过影响细胞因子TNF-α的表达来减少骨吸收。

Objective To observe the effect of Bushen Jianpi Huoxue recipe on the expression of TNF-α in bone tissue of rat postmenopausal osteoporasis model Methods 84 female rats were divided to 7 groups: normal group,sham operation group,model group, Chinese herb control group, western medicine control group, large dose of Bushen Jianpi Huoxue recipe group and small dose of Bushen Jianpi Huoxue recipe group. The rat 's postmenopausal osteoporosis model was made by ovariectomy 90 days after treatment, the expression of TNF-α in bone tissue were be detected. Results Bushen Jianpi Huoxue recipe can decrease the number of TNF-α positive expression cells. The effect of large dose group is superior to small dose Chinese herb control group and western medicine control group. Conclusion The effectiveness of treatment with Bushen Jianpi Huoxue recipe can decrease bone absorption by passing through effecting of the expression of TNF-α.