不同表面活性剂对两性霉素B脂质体体内外性质的影响

Effect of surfactants on the in vitro and in vivo properties of amphotericin B liposome

目的 寻找与DSPE PEG功能相似的表面活性剂修饰两性霉素B脂质体 ,以增加其在体内的稳定性 ,改善其体内分布 ,降低毒副作用。方法 用薄膜超声法制备两性霉素B脂质体 ;比较用DSPE PEG ,Tween 80和Brij35修饰后两性霉素B脂质体包封率、粒径分布、稳定性及组织分布的变化。结果 两性霉素B脂质体的包封率最高为(91 .2± 1 .6) %。修饰后的两性霉素B脂质体包封率提高 ,粒径减小 ,稳定性增加 ,肝、脾和肾中两性霉素B的浓度降低 ,脑中AmB的浓度提高。结论 DSPE PEG和Brij35能提高脂质体逃避网状内皮系统吞噬的能力 ;Tween

Aim Some surfactants such as DSPE-PEG, Tween 80 and Brij 35 were used to modify the amphotericin B liposome, improve the stability, optimize the tissue distribution and decrease the toxicity of am photericin B liposome. Methods The amphotericin B liposome was prepared by the film-supersound method. The effects of cholesterol and amphoter icin B on the encapsulation percentage were studied. The diameter, leakage percentage in phosphate buffer solution(PBS) and calf blood serum, and tissue distributions of...