中药粉膜Ⅱ号体外抗HSV-2作用的实验研究

Experimental Study on Fenmo Ⅱ against Herpes Simplex Virus-2 in vitro

目的:研究中药粉膜Ⅱ号抗 HSV-2作用机理。方法:利用体外细胞培养技术,采用微量细胞病变抑制法来研究中药各浓度组的抑制病毒增殖作用、直接杀伤病毒作用及预防病毒感染细胞作用,本实验以病毒滴度和抑病毒指数为指标。结果:(1)抑制病毒增殖实验:中药粉膜Ⅱ号1.63、0.81、0.41mg/ml 浓度组的病毒滴度与病毒对照组比较,差异有显著性(P<0.01),抑病毒指数>2;(2)直接杀伤病毒实验:中药粉膜Ⅱ号1.63mg/ml—8h 组、1.63mg/ml—24h 组、0.81mg/ml—24h 组的病毒滴度与病毒对照组比较,差异有显著性(P<0.05),其余各组的病毒滴度与病毒对照组比较,差异无显著性(P>0.05);(3)预防作用实验:中药各浓度组的病毒滴度与对照组比较,差异无显著性(P>0.05)。结论:中药粉膜Ⅱ号具有抗 HSV-2病毒的作用,而无预防病毒感染细胞作用。

Objective:To explore the effect and mechanism of Fenmo Ⅱ (FM Ⅱ) against herpes simplex virus-2 (HSV-2) for treatment of cervical erosion.Methods:Using micro-inhibition cytopathic and in vitro cell culture technique,the various concentrations of FM Ⅱ in inhibiting HSV-2 proliferation and the direct virucidal effect to prevent HSV-2 in infecting cells were studied.The virus titer and virus inhibition index were taken as parameters.Results:(1)Virus proliferation inhibition experiment:Compared with control grou...