摘要
目的:探讨内关穴在心肌缺血再灌注损伤过程对心肌细胞凋亡的影响。方法:采用电针针刺家兔左右侧内关穴20min 后,结扎左冠状动脉前降支,ECG 监测,40min 后恢复灌流,再灌注1h,取心肌组织,透射电镜及原位末端标记(TUNEL)法观察凋亡细胞的变化。结果:缺血再灌注模型组出现典型的凋亡细胞,棕褐色标记产物广泛存在;针刺内关组染色质边集均不显著,棕褐色标记产物明显减少。结论:电针内关可降低缺血再灌注损伤的程度,对心肌细胞的凋亡有明显的抑制作用。
Objective:To explore the influence of electroacupuncturing(EA)Neiguan point on cell apoptods in myocardial ischemia/reperfusion injury.Methods:After EA Neiguan point had been applied for 20 min,the left anterior descending coronary artery of rabbit was ligated for 40 min and reperfused for 1 hr under ECG monitoring.Myocardium was then taken for observing the changes of cell apoptosis by transmission electron microscopy and TUNEL.Results:Typical apoptotic cells and dark brown marked products appeared extensi...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2002年第S1期25-28,共4页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
针刺内关
缺血再灌注损伤
细胞凋亡
原位末端标记法
acupuncturing Neiguan point
ischemia/reperfusion injury
cell apoptosis
terminal deoxynucleotidyl transferse mediated uridine nucleotide end labeling
