连续4次(每次间隔36h)部分肝切除对大鼠肝ACP、AKP、HSC70/HSP68和PCNA的影响

EFFECTS OF FOUR TIMES (INTERVAL 36 h) SUCCESSIVE PARTIAL HEPATECTOMY ON THE ACTIVITY AND CONTENT OFACP, AKP, HSC70/HSP68 AND PCNA IN RAT LIVER

在分析了连续 3次 (每次间隔 4h)部分肝切除对肝细胞生化和增殖的影响后 ,本文又以连续 4次 (每次间隔 3 6h)部分肝切除 (shortintervalsuccessivepartialhepatectomy ,SISPH)大鼠为模型 ,分析了SISPH对肝脏酸性磷酸酶 (ACP)、碱性磷酸酶 (AKP)、构成性热休克蛋白 70 /诱导性热休克蛋白 68(HSC70 /HSP68)和增殖细胞核抗原 (PCNA)活性和含量的影响。结果表明 ,第 1次切除肝的左外叶后恢复 3 6h ,ACP和AKP活性及HSC70 /HSP68和PCNA表达量均增加 ;第 2、 3、 4次分别切除左中叶和中叶、右叶、尾状叶 (每次间隔 3 6h)后 ,AP活性和PCNA含量与AKP活性和HSC70 /HSP68含量呈负相关性 ;间隔 3 6h的连续部分肝切除延缓了细胞分化时间。根据上述实验中ACP和AKP在细胞内位置和活性变化推测 :AKP和ACP可能共同参与了细胞的增殖启动 ;PCNA和AKP可能参与了DNA合成和细胞增殖 ;ACP和HSC70 /HSP68可能在蛋白质转运、选择性降解和细胞结构、功能重建中起重要作用。

In the previous paper, we reported the effects of three time (interval 4 h) successive partial hepatectomy (SPH) on the activity and content of the acid phosphatases (ACP), alkaline phosphatases (AKP), constitutive heat shock protein70/induced heat shock protein68 (HSC70/HSP68) and proliferating cell nuclear antigens (PCNA) during rat liver regeneration. In this paper, we studied their changes in four times (interval 36 hr) SPH. The results showed: (1) the activity and content of ACP, HSC70/HSP68 AKP and PC...