陈旧性骨骼DNA提取技术的研究

A study on the extraction method DNA from the aged bone

对陈旧性骨骼建立一个高回收率并能除去 PCR 反应抑制物的提取 DNA 的方法。采用 CTAB 法提取 DNA。结果显示,该方法不但能有效去除 PCR 抑制物,而且对水泡、火烧、土埋以及10年左右的骨骼所提取的 DNA 均能成功地进行荧光标记 STR 多基因座扩增检验。实验表明,该方法稳定,重复性好,适合陈旧骨骼标本的 DNA 提取。

The STR multiplex method is not efficient in amplifying DNA samples from the aged bones due to the degradation of the DNA extracted and the presence of PCR inhibitors in the collagens of the aged bones.For the purpose of solving this problem,the CTAB method has been established to extract DNA from bones previously soaked into the water,burned and buried for approximately 10 years.The CTAB method was effective in removing the PCR inhibitors from the aged bones and then the DNA were success- fully genotyped b...