高效液相色谱法测定饲料中土霉素

Determination of Oxytetracycline in Feed by HPLC

建立了高效液相色谱测定饲料中土霉素含量的方法。土霉素在0.1~50μg/mL范围内呈现良好的线性关系,相关系数r为0.999 9;检出限为0.5 mg/kg,定量限为2 mg/kg;回收率为94.4%~98.6%。实验结果表明该法准确度高、重现性好、结果可靠。

A HPLC method to determine the contents of oxytetracycline in feed was established.The concentration of oxytetracycline and peak area was linear correlation between 0.1~50 mg/kg,correlation coefficient r=0.999 9.The limit of detection was 0.5 mg/kg,the limit of quantification was 2 mg/kg;the reclaim ratio was 94.4%～98.6%.The results indicated that the constructed method is accurate,repeatable and reliable.