摘要
利用RAPD标记技术对以"pt"型雄性不育育成的胡萝卜杂交种"金红四号"及其父、母本进行DNA多态性分析,从分子水平上探寻鉴定胡萝卜杂交种的方法。结果表明,从40条随机引物中筛选出具有特异性扩增带的引物4条,利用这些特异性扩增带能有效的区分和鉴定"金红四号"及其父、母本。说明利用RAPD方法对"金红四号"胡萝卜杂交种及其父、母本进行快速、准确的鉴定是可行的。
The RAPD analysis was conducted on genome DNA from the "pt" cytoplasm male sterility type carrot Jinhong No.4 hybrid and its parents,In order to seek the method of identifying carrot hybrid.4 primers which amplified respectively bands can distinguish F1 hybrid of Jinhong No.4 and its parents screened from 40 random primers.This results indicated that RAPD analysis could be a rapid and reliable method to identify carrot hybrid.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2008年第2期12-14,共3页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
内蒙古自然科学基金资助项目(200508010306)
