六味地黄丸对2型糖尿病大鼠胰岛形态的影响

Therapeutic Effects of Liuwei Dihuang Wan on Type 2 Diabetes Rats Model

目的:研究六味地黄丸对高糖高脂饲料喂养联合低剂量链脲佐菌素(streptozotocin,STZ)注射的2型糖尿病大鼠胰岛形态的影响。方法:高糖高脂饲料喂养5周后腹腔注射低剂量的STZ(40mg/kg)制备大鼠2型糖尿病模型。予六味地黄丸水煎剂治疗3周,动态观察治疗后的饮食量、体重、空腹血糖(FBG)变化,3周末检测血清游离脂肪酸(FFA)、甘油三酯(TG)、血清胰岛素(INS)水平,计算胰岛素敏感性指数(ISI),并在光镜和透射电子显微镜下观察胰腺形态学变化。结果:与模型组比较,六味地黄丸组在用药2周末饮水量明显减少,3周末进食量减少,FBG出现降低的趋势,FFA降低,ISI显著升高,形态学显示胰岛β细胞数量增多,细胞内分泌颗粒丰富,胰岛周边部导管上皮样细胞增多,并向胰岛中央移行。结论:六味地黄丸对高糖高脂饲料喂养联合低剂量STZ注射的2型糖尿病大鼠胰岛结构具有保护作用。

Objective:To investgate the effects of Liuwei Dihuang Wan on morphological change of pancreas islets in type 2 diabetes rats imodel. Methods:SD male rats were used to establish Type 2 diabetes rat model by injecting with streptozotocin(STZ) 40mg/kg after feeding with high-fat, high-sucrose diet for 5 weeks. Then the model rats were randomly divided to treat with Liuwei Dihuang Wan or vehicle orally for 3 weeks. FBG, body weight, food and water intake were monitored weekly. Free fatty acid (FFA), triglycerid...