气相色谱分析生地黄多糖的单糖组成及其含量

Analysis of monosaccharides in Radix Rehmanniae by GC

目的:对生地黄多糖进行分离纯化,并进行组成分析。方法:经沸水抽提、乙醇沉淀、三氯乙酸脱蛋白,再经Superdex 200和Sephadex G100凝胶色谱得到纯化的生地黄多糖SRPⅠ和SRPⅡ,经凝胶色谱分析,均为单一多糖。经水解、衍生化后,用气相色谱分析其多糖的组分。结果:经分离纯化,从生地黄中得到了2个单一的纯品多糖SRPⅠ和SRPⅡ。结论:生地黄多糖SRPⅠ是由鼠李糖、阿拉伯糖、葡萄糖和半乳糖等单糖组成,其质量分数分别为6.11%,66.46%,3.93%和21.50%;而生地黄多糖SRPⅡ是由鼠李糖、岩藻糖、甘露糖、半乳糖和果糖等单糖组成,质量分数分别为21.82%,24.47%,10.48%,29.94%和13.29%。

Objective:To isolate and purify the polysaccharides from Radix Rehmanniae and analysis the monosaccharides composition.Method:The polysaccharides were extracted with hot water and precipitated by alcohol.Proteins in the precipitates were removed by TCA method.The products were further purified with column chromatography on Superdex 200 and Sephadex G100.The SRPⅠ and SRPⅡ were identified as homogeneous polysaccharide by HPLC,respectively,and then analysised by GC after being hydrolysised.Result:Two homogeneo...