中药复方抑瘤饮对小鼠S180移植瘤血管生成的动态研究

Dynamic study on effect of Chinese medicine compound Yiliuyin on angiogenesis in transplanted S180 tumor of mouse

目的:研究荷S180瘤小鼠灌服中药复方抑瘤饮不同时间点肿瘤内血管生成的动态变化,揭示抑瘤饮体内抗瘤的作用机制。方法:56只BALB/c小鼠随机分为抑瘤饮组和对照组(n=28),每组分为4个亚组(每亚组7只)。右腋皮下接种S180肿瘤细胞24 h后,抑瘤饮组小鼠灌服抑瘤饮,对照组小鼠灌服等量凉开水,每日2次,每次0.5 mL;2组分别于灌服第10,20,30,40天点各处死一亚组小鼠。分离肿瘤制成石蜡包埋切片,免疫组化法检测肿瘤组织内血管生成(CD34染色)、血管内皮生长因子(VEGF)、血管内皮生长因子受体-2(VEGFR-2)和内皮抑素(ES)的动态变化,结果以阳性酶点(positive enzyme dot,PED)表示。结果:抑瘤饮能显著降低肿瘤组织内微血管生成:抑瘤饮组CD34在第10,20,30,40天点PED均低于对照组(分别P<0.05,P<0.01,P<0.01和P<0.01)。抑制VEGF和VEGFR-2的表达:抑瘤饮组VEGF在第10,20,30,40天点PED均低于对照组(分别P<0.05,P<0.01,P<0.01和P<0.01):抑瘤饮组VEGFR-2在相应时间点均低于对照组(分别P<0.05,P<0.01,P<0.01,P<0.01)。促进ES的表达:抑瘤饮组ES在相应时间点与对照组比第10天点PED无明显差异,在第20,30,40天点PED均高于对照组(分别P<0.05,P<0.05和P<0.01)。结论:抑瘤饮体内可通过下调VEGF和VEGFR-2表达,上调ES表达,抑制肿瘤组织内血管生成发挥抗瘤作用。

Objective:To investigate the dynamic changes in angiogenesis within the tumor tissue of mice bearing S180 tumor at different day-points of oral administration with a Chinese medicine compound"Yiliuyin"(YLY) and to explore the anti-tumor mechanisms of YLY in vivo.Method:Fifty-six BALB/c mice were divided into YLY group and control group (28 mice/group) and each group was divided into four subgroups (7 mice/subgroup),randomly.After 24 hrs of inoculation with S180 tumor cells subcutaneously in the right axilla...