高效液相色谱法测定复方丹参片丹参酮ⅡA含量

Determination of tanshinone ⅡA in compound tanshin tablet by HPLC

目的 :建立测定复方丹参片中丹参酮ⅡA含量的高效液相法。方法 :色谱条件 :色谱柱SpherisorbC1 8(5 μ ,2 0 0mm× 4.6mm)色谱柱 ;流动相 :甲醇 -水 (85∶1 5 ) ;检测波长 :2 70nm ;流速 :1ml/min。结果 :丹参酮ⅡA在 8～ 1 80ng范围内线性关系良好 (r=0 9999) ,最低检出限 1 5ng ,阴性样品对测定无干扰 ,溶液在 2 4h内保持稳定 ,加样回收率 95 %以上。结论 :该方法快速、准确 ,可用于复方丹参片中丹参酮ⅡA的鉴别和含量测定。

OBJECTIVE:To develop a method of HPLC to determine the content of tanshinone ⅡA in compound tanshin tablet,METHODS: Spherisorb C 18 (5μ,200mm×4.6mm)was used,mobile phase wasmethanol-waver(85∶15).The detection wanelength was 270nm.RESULTS:The linear ranges of determination of tanshinone ⅡA was 8～180ng(r=0.9999),the average recovery rate was 99.5%.CONCLUTION:This method may be used for determination tanshinone ⅡA in compound tanshin tablet.