原花青素对2型糖尿病局灶性脑缺血大鼠脑组织SOD和NO的影响

Effect of Procyanidin on Expression of SOD and NO in Type 2 Diabetes Mellitus Rats with Focal Cerebral Ischemia

目的探讨原花青素(procyanidin,PC)对2型糖尿病局灶性脑缺血大鼠脑组织中超氧化物歧化酶(SOD)和一氧化氮(NO)表达的影响。方法将60例大鼠随机分为假手术组、2型糖尿病单纯脑缺血组及PC低剂量组(50mg/kg)、中剂量组(100mg/kg)及高剂量组(200mg/kg),于脑缺血后24小时进行神经功能评分及HE染色,应用黄嘌呤氧化酶法测定脑组织SOD活性,硝酸还原酶法测定脑组织NO含量。结果假手术组大鼠脑组织SOD活性为(60.37±2.04)U/mg,NO含量为(13.55±1.43)μmol/L。与之相比,模型组SOD活性[(46.53±1.00)U/mg]减少(P<0.01),NO含量[(22.66±3.07)μmol/L]增多(P<0.01);与模型组相比,PC低剂量组大鼠脑组织中SOD活性[(48.75±0.98)U/mg]升高(P<0.05);与PC低剂量组相比,PC中剂量组和PC高剂量组大鼠脑组织中SOD活性分别为(56.20±1.47)U/mg,(56.73±1.14)U/mg,差异均有统计学意义(P<0.01)。与模型组相比,PC低剂量组大鼠脑组织中NO含量([20.03±1.21)μmol/L]升高,差异有统计学意义(P<0.05);与PC低剂量组相比,PC中剂量组和PC高剂量组大鼠脑组织中NO含量下降,分别为(17.34±0.61)μmol/L,(16.97±0.46)μmol/L,差异均有统计学意义(P<0.01)。结论原花青素可能通过增加SOD的活性和降低NO的含量对2型糖尿病局灶性脑缺血大鼠起到一定的保护作用。

Objective To investigate the effect of procyanidin(PC) on expression of superoxide dismutase(SOD) and nitric oxide(NO) in type 2 diabetes mellitus SD rats with focal cerebral ischemia. Methods Sixty SD rats were divided into sham-operation, model, low-dose PC, mid-dose PC and high-dose PC group. After ischemia for 24h, we evaluated the neurological function of SD rats, performed hematoxylin and eosin (HE) staining, then used xanthinoxidase method to measure the activity of SOD and nitrate reducase method to measure the level of NO. Results The activity of SOD in rats’ brain of model group decreased signif icantly when compared with sham-operation group[(46.53±1.00)U/mg vs (60.37±2.04)U/mg, P<0.01], while the level of NO increased signifi cantly[(22.66±3.07)μmol/L vs (13.55±1.43)μmol/L, P<0.01]. Compared with model group, the activity of SOD in rats’ brain of low-dose PC group signifi cantly increased[(48.75 ±0.98) U/mg, P<0.05] and the level of NO decreased signifi cantly[(20.03±1.21)μmol/L, P<0.05]. When compared with low-dose PC group, the activity of SOD in mid-dose and high-dose PC group signifi cantly increased [ (56.20±1.47)U/mg and (56.73±1.14)U/mg respectively, P<0.01] and the level of NO decreased significantly[(17.34±0.61)μmol/L and (16.97±0.46)μmol/L respectively, P<0.01]. Conclusion Procyanidin has the neuroprotective effect on ischemic rats of type 2 diabetes mellitus by increasing the activity of SOD and decreasing the level of NO.