蛇床子素在老年性痴呆模型大鼠血浆的药代动力学研究

Pharmacokinetics of osthol in plasma in rats of Alzheimer's Disease model

目的研究蛇床子素在老年性痴呆模型大鼠血浆的人体药代动力学参数。方法对D-半乳糖致亚急性衰老合并鹅膏蕈氨酸损毁Meynert核的AD大鼠模型,以40mg/kg的剂量灌胃蛇床子素,采用高效液相色谱法测定大鼠的蛇床子素血药浓度。结果蛇床子素的体内动态过程呈二房室开放模型,Cmax为(2.742±0.83)μg/ml,tmax(0.56±0.18)h,MRT(6.03±3.2)h,t(5.26±2.6)h,AUC0-24(10.62±4.65)μg·h/ml,AUC00-∞(11.22±4.15)μg·h/ml。结论此方法简便、准确、重现性好,可用于蛇床子素血药浓度分析及药代动力学研究。

Objective To study the pharmacokinetics of osthol in rats of Alzheimer's Disease model.Methods A HPLC method was established for the determination of osthol in plasma after a single oral dose of 40mg/kg osthol in D-galacose and IBO induced aging rats model.Results The osthol plasma concentrations conformed to a two-compartment open model and the main pharmacokinetic parameters were as follows:Cmax were(2.742±0.83)μg/ml,tmax were(0.56±0.18)h,MRT were(6.03±3.2)h,t were(5.26±2.6)h,AUC0-24 were(10.62±4.65)μg·h/ml,AUC0-∞were(11.22±4.15)μg·h/ml.Conclusions his method is simple,accurate and reproducible and has been applied successfully to the determination of concentration of osthol.