广西藤茶提取物APS体外抗菌作用研究

Antibacterial effects for APS from Tengcha in vitro

目的: 观察从广西藤茶中提取得到的一个单体化合物(简称APS) 的体外抗菌作用。方法: 利用琼脂稀释法对标准金黄色葡萄球菌(S. aureus) 、耐甲氧西林金黄色葡萄球菌( MRSA) 、绿脓杆菌( P.aeruginosa) 、大肠杆菌(E.coli) 、乙型溶血性链球菌( β-hemolytic streptococcus) 、福氏痢疾杆菌( Shigella flexneri) 、白色葡萄球菌(Staphylococcus albus)、奈氏菌(Neisseria)、白色念珠菌(Candida albicans)、枯草杆菌(Bacillus subtilis)进行最低抑菌浓度( MIC) 测定, 并与盐酸黄连素相比较, 利用不同浓度 APS 对标准金黄色葡萄球菌、MRSA、绿脓杆菌进行体外杀菌曲线测定。结果: 对 S. aureus、MRSA、P. aeruginosa、E.coli、β- hemolytic strepto-coccus、Shigella lexneri、Staphylococcus albus、Neisseria、Candida albicans、Bacillus subtilis 的 MIC 值分别为 0.078、0.078、1.25、0.31、2.5、1.25、0.625、2.5、5、1.25mg/mL; 体外杀菌曲线表明, APS 对 S. aureus、MRSA、P. aeruginosa 有直接抑制作用, 且与药物剂量呈正相关。结论: APS 体外具有明显的抗菌作用。

Objective: To investigate the antibacterial effects of APS in vitro, a monomer extract from the Chinese herb Ampelopsis grossedentata. Methods: Compared with berberine chloride, MIC were determined by the agar dilution methodology;the antibacterial activities of APS against Staphylococcus aureus (S.aureus)?methicillin-resistant Staphylococcus aureus(MRSA) and Pseudomonas aeruginosa (P.aeruginosa) were determined by a time-effect study. Result:APS had good antibacterial activities against S.aureus, MRSA, P.aeruginosa,E.coli,β-hemolytic streptococcus,Shigella flexneri,Staphylococcus albus,Neisseria,Candida albicans,Bacillus subtilis, and MICs were 0.078,0.078,1.25,0.31,2.5,1.25,0.625,2.5,5,1.25mg/mL, respectively.Bactericidal curve show that APS has inhibitory effect against Staphylococcus aureus, MRSA and P.aeruginosa. Conclusion: APS has antibacterial effects in vitro.