摘要
基于人血清白蛋白(HSA)对邻苯二酚紫的共振散射的增强效应,拟定了一种新的测定HSA的共振散射法.在pH=4.20的条件下,邻苯二酚紫只有极弱的光散射,但它与蛋白质的结合物却有强烈的共振散射作用,在λ=562nm处,光散射有最大的散射强度,并且光散射强度与蛋白质HSA的浓度成线性关系,线性范围为0.0～10.0mg/L,该法简便,快速,用于人血清样品蛋白质的测定,并与用CPA-pA-Ba法测定的结果相比较,结果令人满意.
A new Resonance light scattering (RLS) method is presented in this paper.In pH 4.20 phosphate buffer,catechol violet combined with proteins by intermolecular forces resulted in an enhanced Resonance light scattering.The RLS intensity at 562 nm is maximum and proportional to the concentration of protein in the range of 0.0～10.0 mg/L.The method is simple,rapid and sensitive.It has been applied to the determination of protein in human serum sample with satisfactory results.
出处
《广西师范大学学报（自然科学版）》
CAS
2004年第2期55-58,共4页
Journal of Guangxi Normal University:Natural Science Edition
基金
广西教育厅科学研究基金资助项目(桂教科研[2003]22号)
关键词
共振散射
蛋白质
邻苯二酚紫
含量
测定
Resonance light-scattering
protein
catechol violet
concentration
determination
