同时测定参附注射液二组份含量方法学研究

Determination of two components in ginseng and monkshood parenteral solution at one time

目的:建立同时测定参附注射液中人参皂苷Rg1和人参皂苷Re的含量方法。方法:高效液相色谱法,Nova Pak C18柱,流动相:乙腈:水(70:30),检测波长:203nm。结果:人参皂苷Rg1和人参皂苷Re分别在0.7208μg-7.208μg(r=0.9997,n=6)和0.6032μg-6.032μg(r=0.9997,n=6)范围内具有良好的线性关系。加样回收率分别为99,6％(RSD=1.2％)和99.8％(RSD=1.3％)。结论:该方法准确,灵敏,便捷,可同时控制参附注射液中人参皂苷Rg1和人参皂苷Re的含量。

Objective: To set up quantitative high performance liquid chromatographic method for the simultaneous determination of Ginsenoside Rg1 and Ginsenoside Re in ginseng and monkshood parenteral solution. Method: An RP - HPLC method was set up,using Nova Pak C18 column, ace-tontrile: water(70: 30) as mobile phase with a flow rate of 1.0mL/min at the detection wavelength of 203nm. Results: The calibration curves were separately linear of Ginsenoside Rg1 and Ginsenoside Re between 0. 7208μg - 7. 208 μg( r = 0. 9997, n = 6)and 0. 6032μg - 6. 032 μg( r = 0. 9997, n = 6); theaverage recoveries of Ginsenoside Rg1 and Ginsenoside Re were separately 99. 6% (RSD = 1. 2% )and 99. 8% (RSD = 1. 3% ). Conclusion: This method is simple, quick, reproducible and is suitable for the quality control of two Ginsenosides in ginseng and monkshood parenteral solution at One Time.