一测多评法同时测定黄芩药材中4种黄酮类成分的含量

A quantitative method for simultaneous assay of four flavones with one marker in Radix Scutellariae

目的:建立黄芩药材一个对照品同步测定4个成分的分析方法,并建立其方法学的考察模式。方法:采用HPLC-DAD以药材中典型成分黄芩苷、汉黄芩苷、黄芩素、汉黄芩素为指标,在一定的线性范围内,建立黄芩苷与后3种成分间的相对校正因子(分别为0.746 7,0.563 8,0.435 1),并测定黄芩苷的含量,用所建立的校正因子计算其他3个成分的含量;同时采用外标法测定27批药材中以上4种成分的含量;以验证一测多评法的准确性和科学性。结果:建立的校正因子重现性良好,黄芩药材中采用校正因子计算的含量值与外标法实测值之间没有明显差异。结论:在对照品缺乏的情况下,该方法可以作为一个新模式用于药材的多成分定量。

Objective: To develop a quantitative method for simultaneously determining four flavones in Radix Scutellariae,by using one chemical standard substance.Method: The relative correction factors(RCF) of four flavones were determined by HPLC-DAD.Within the linear ranges,the values of RCF at 274 nm of wogonoside,baicalein,and wogonin to baicalin,were 0.746 7,0.563 8,0.435 1,respectively.And the contents of baicalin in 27 samples of Scutellaria baicalensis were authentically determined by the external standard me...