人工加速老化处理对小麦种子生理生化特性的影响

Effect of Artificial Aging on Physiological and Biochemical Characteristics of Wheat Seeds

为探明小麦种子贮藏过程中的生理生化变化及种子活力下降的主要原因,采用高温高湿人工加速老化的方法,在90%RH(相对湿度)和55℃条件下对非糯小麦及糯小麦种子进行老化处理,测定其发芽率、电导率、丙二醛含量、可溶性糖含量、SOD、POD、CAT活性和可溶性蛋白质含量的变化。结果表明,种子发芽率随老化处理时间的延长而逐渐降低,且在相同处理时间下非糯小麦种子的发芽率都高于糯小麦;种子浸出液电导率则随处理时间延长呈微升趋势,在整个处理过程中非糯小麦种子浸出液电导率均低于糯小麦;非糯小麦POD活性、SOD活性总体上明显高于糯小麦种子,而可溶性糖、可溶性蛋白质和丙二醛含量则低于糯小麦。非糯小麦种子的抗老化能力显著强于糯小麦种子。

A study on the physiological and biochemical changes during storing and its main reasons causing vigor descent of wheat seeds were conducted to further explore the process of seed deterioration.The Waxy and non-waxy wheat seeds were selected and then artificial aged under 55 ℃ and 90%RH,the physiological and biochemical indexes,such as germination rate,conductivity,Malondialdehyde(MDA) content,soluble sugar content,soluble protein content,Superoxide dismutase(SOD) activity,Percxidase(POD) activity,and Catal...