几个供体对优质蛋白玉米(QPM)近等基因系构建效果的比较

Comparison of Several Donor Inbred Lines on Development of Quality Protein Maize(QPM) Near-isogenic Lines

对CA335、CA339、山东2548和齐205这4个国内常用优质蛋白玉米自交系的赖氨酸含量、子粒表型、醇溶蛋白积累及opaque-2基因的序列进行分析,对不同自交系作为opaque-2突变基因(o2)基因供体的分子标记选择效果进行比较。结果表明,4个自交系属于两类不同的o2突变体类型,CA335和CA339为一类,山东2548和齐205为一类。两类突变体作为o2供体选择效果有较大差异,CA335和CA339是优良供体,齐205和山东2548则不是理想供体。对于优质蛋白玉米育种,供体的选择非常重要,好的QPM自交系不一定是好的供体。

Lysine content,kernel phenotype,zein accumulation and opaque-2 gene sequence of four maize inbred lines(CA335,CA339,Lu2548 and Qi205) were investigated in this study.These QPM inbred lines as the donor of opaque-2 mutant gene(o2) respectively,were backcrossed to introgression o2 into elite maize inbred lines based on marker-assisted.The results showed that these four QPM inbred lines belonged to two different types of o2 mutant,CA335 and CA339 were the first type,and Lu2548 and Qi205 were another type.There...