紫外分光光度法测定更辰胶囊中谷维素的含量

Determination of the Content of Oryzanol in Gengchen Capsules by UV Spectrophotometry

目的:建立更辰胶囊中谷维素的含量测定方法。方法:以正庚烷为提取溶剂,直接提取制剂中谷维素,以正庚烷作空白,在波长314.6nm处用紫外分光光度法测定谷维素的含量。结果:谷维素的质量浓度在0.10mg/ml～0.60mg/ml范围内与吸光度呈良好的线性关系,r=0.9998,平均回收率为100.505%,RSD为0.81%(n=6)。结论:该法简便、准确、重复性好,可作为该制剂中谷维素的含量测定方法。

Objective:To establish the determination method of Oryzanol in Gengchen Capsules.Methods:Skellysolve C was used as extraction solvent,the content of Oryzanol was determined directly at 314.6nm by UV spectrophotometry.Results:The absorbance has a linear relationship with Oryzanol with the range of 0.10mg/ml～0.60mg/ ml,r=0.9998.The average recovery rate was 100.505%,and RSD was 0.81%(n=6).Conclusion:The method is simple, accurate and has good reproducibility.It can be for the assay of Oryzanol in the preparation.