胶体金法、免疫荧光法检测血清肌钙蛋白I的比较

A Gold law,Immune Fluorescence Detection Method the Serum Proteins Ⅰ Detected a Comparison of Calcium

目的:通过两种方法检测结果的比较,对胶体金法(Colloidal Gold Assay)检测血清心肌肌钙蛋白I(cT-nI)进行方法学评价,并探讨其临床应用价值。材料与方法:应用胶体金法测定20名正常人和20例急性心肌梗死(AMI)患者的血清cTnl水平,并与免疫荧光法(IFA)检测结果相比较,系统研究胶体金法测定cTnI的敏感性、特异性、可用度。结果:胶体金法(Y)与免疫荧光法(X)具有良好的相关性(Y=0.801X-0.174,r=0.980),卡方检验P>0.05,两种方法结果无显著性差异。诊断敏感度、特异度、可用度分别为85%、95%、80.3%,阳性和阴性似然比分别为17和0.158,阳性预测值和阴性预测值分别为94.4%、86.4%。结论:胶体金法操作简便、结果准确可靠、自动化程度高,且检测快速,具有较高的临床可用度;cTnI对诊断AMI有高度特异性和敏感性,且持续时间较长,适合在临床工作中推广应用。

Purpose:By the comparison of two methods,to evaluate the analytical features of the new automated cardiac troponin I(cTnl) assay using colloidal gold assaytechnology and to obtain information about lt 's utility in clinical studies.Materials and Methods:cTnI concent ration was determined in 20 healthy individuals and 20 patients with AMI,thecomparison study was also performed versus Immunofluoresccnce assay.The analyticalsensitivity,specificity,availability of the cTnl assay were determined.Results:Linearregression analysis showed a good correlation between the colloidal gold assay(Y) andinmunofluorescence assay(X) methods(Y=0.801X-0.174.r=0.980).There is nosignificant difference(P>0.05)between two methods.The sensitivity,specificity,availability,positive likelihood ratio,negative likelihood ratio,positive predictive value(PPV) and negative predictive value(NPV) were 85%,95%,80.3%,17,0.158,0.944 and0.864 respectively.Conclusion:The colloidal gold method is reliable,available,fairly rapid and easy to perform.cTnl is an excellent marker for detecting and ruling out AMI,because ithas high specificity and sensitivity,and lasts for a long time This method is very useful andsuitable for routine determinations of serum cTnl levels in clinical laboratory.