消瘀理冲汤对慢性输卵管炎性大鼠IL-6与TNF-α的影响

Effect of Xiaoyu Lichong Decoction on IL-6 and TNF-α of Rats with Chronic Salpingitis

[目的]验证消瘀理冲汤治疗慢性输卵管炎性不孕的疗效并探讨其作用机制。[方法]将59只雌性SD大鼠随机分为正常对照组、模型对照组、桂枝茯芩丸组、消瘀理冲汤低剂量组、消瘀理冲汤高剂量组、康妇炎胶囊组,按设计给药。采用SABC法做输卵管组织白细胞介素-6(IL-6)的免疫组化及肿瘤坏死因子-α(TNF-α)的原位杂交分析。[结果]消瘀理冲汤组的输卵管组织IL-6表达明显低于其他组(P<0.01);TNF-α表达由于出现了部分非特异性表达导致了各治疗组差异性不大(P>0.05),但治疔组与模型组之间有显著差异(P<0.01)。[结论]消瘀理冲汤能降低慢性输卵管炎性大鼠IL-6和TNF-α的表达,对慢性输卵管炎具有显著治疗作用。

[Objective]To testify the cure effect of Xiaoyu Lichong Decoction on chronic salpingitis sterility and discuss the mechanism.[Method] Randomly divide 59 female SD rats into normal control group(l),model controI(2),Cassia Twig and Fuling Pill group(3),low(4),middle(5) and high dosage(6) groups of Xiaoyu Lichong Decoction,and Kangfuyan Capsule group(7),administer drugs respectively;take SABC method to make in-situ hybridization analysis on IL-6 and TNF-α.[Result]Groups 4,5 and 6 had lower expression than other groups;on TNF-a,there's much difference between treatment groups and model groups.[Conclusion]Xiaoyu Lichong Decoction can reduce IL-6 and TNF-a expression of chronic sapingitis rats and treat the disease.