高效液相色谱法测定乳制品中纳他霉素的不确定度评定

Uncertainty Evaluation for HPLC Determination of Natamycin in Dairy Products

依据JJF 1059—1999《测量不确定度评定与表示》的原理与方法,建立高效液相色谱法测定乳制品中纳他霉素结果不确定度评定的数学模型。对整个测量过程的不确定度来源进行分析,并对不确定度各个分量进行评估和合成,得出合成标准不确定度为0.014,扩展不确定度为0.28mg/kg,乳制品中纳他霉素测定结果的置信区间为(9.95±0.28)mg/kg,k=2。

According to the principle and procedure of JJF1059—1999 Evaluation and Expression of Uncertainty in Measurement,a mathematical mode was established for uncertainty evaluation for HPLC determination of natamycin in dairy products.Sources of uncertainty in the entire measurement process were analyzed and each component of uncertainty was evaluated and synthesized.The synthetic standard uncertainty was 0.014,and the expanded uncertainty was 0.28 mg/kg.The confidence interval for natamycin determination was(9.95 ± 0.28) mg/kg,k = 2.