牛乳β-乳球蛋白过敏原表位及其潜在应用

Epitopes on bovine β-lactoglobulin and its potential application

过敏原表位是过敏原直接参与免疫反应的物质基础,也是导致过敏反应的"元凶"。牛乳β-乳球蛋白是lipocalin家族的代表和常用模式蛋白,它的表位信息比较完整,拥有7个人血清IgE和6个IgG的线性表位、3个IgE结合的构象性表位以及7个T细胞表位。另外,它的动物源血清IgE、IgG和T细胞表位也在不断被发现。牛乳β-乳球蛋白的表位信息为基于表位的应用提供了重要的支撑,它们的应用主要涉及到预测食物过敏原的致敏性,食物过敏的交叉反应,食物过敏的诊断,食物过敏的免疫治疗以及食物过敏原的检测等5个方面。

Epitopes is the binding sits between allergen and the relevant antibody,and it is the triggering element of immune reaction.Bovine β-lactoglobulin belongs to lipocalin family and is one of the model proteins.It was found that 7 IgE binding and 6 IgE binding linear epitopes,3 IgE binding conformational epitopes and 7 T cells binding epitopes existed in milk allergy patients,and the binding epitopes of other animal resource were also reported.The epitopes information plays a very important role in the application based on epitopes,and the application includes the prediction of food allergen allergenicity,cross-reactivity of food allergen,diagnosis of food allergy,immunotherapy of food allergy and analysis of food allergen.